Biolegend, 127625, Alexa Fluor® 488 anti-mouse Ly-6G Antibody
25microg
Catalog Number: 127625
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Pack Size6 g
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Ly-6G transfected EL-4J cell line. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662). |
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| Application References(PubMed link indicates BioLegend citation) | Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Barkaway A, et al. 2021. Immunity. . PubMed Sweet R, et al. 2017. J Immunol. 10.4049/jimmunol.1600861. PubMed Dong X, et al. 2019. ACS Nano. 13:1272. PubMed Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed El-Naccache DW, et al. 2022. Cell Rep. 40:111150. PubMed Abdelnabi MN, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1269. PubMed Lee DE, et al. 2022. Nat Commun. 13:7613. PubMed Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed Cagle LA, et al. 2023. Front Physiol. 13:1059686. PubMed Gershater M, et al. 2022. J Immunol. 208:1595. PubMed Liu S, et al. 2020. Neuron. S0896-6273(20)30532-8.. PubMed Joy MT, et al. 2019. Cell. 176:1143. PubMed Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed Jahan S, et al. 2022. J Biol Chem. :102386. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Stolp B, et al. 2022. Cell Rep. 38:110387. PubMed Craver BM, et al. 2020. Blood Adv. 4:312. PubMed Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed Leung H, et al. 2022. Nat Commun. 13:5206. PubMed Green D, et al. 2020. Oncogene. 39:5553. PubMed Wu L, et al. 2022. Theranostics. 12:842. PubMed Souza COS, et al. 2021. iScience. 24(6):102548. PubMed Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed Myerson JW, et al. 2021. Nat Nanotechnol. Online ahead of print. PubMed Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed Pach E, et al. 2021. Cancers (Basel). 13:. PubMed Du J, et al. 2021. Mol Med Rep. 23:. PubMed Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed Liu G, et al. 2022. Ann Transl Med. 10:693. PubMed Takizawa S, et al. 2022. Mol Med. 28:91. PubMed Moon H, et al. 2019. Nat Commun. 10:2225. PubMed Pinho–Ribeiro FA, et al. 2018. Cell. 173:1083. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Boras M, et al. 2017. J Exp Med. 214:851. PubMed |
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| RRID | AB_2561339 (BioLegend Cat. No. 127625) AB_2561340 (BioLegend Cat. No. 127626) |
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| Structure | A 21-35 kD GPI-anchorded membrane protein |
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| Distribution | Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C. |
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| Cell Type | Granulocytes, Macrophages, Monocytes |
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| Biology Area | Immunology, Innate Immunity |
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| Antigen References | Fleming TJ, et al. 1993. J. Immunol. 151:2399. |
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| Gene ID | 546644 |
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| UniProt | View information about Ly-6G on UniProt.org |
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| Clone | 1A8 |
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| Regulatory Status | RUO |
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| Other Names | Lymphocyte antigen 6 complex, locus G |
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| Isotype | Rat IgG2a, κ |
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