Biolegend, 135017, Alexa Fluor® 488 anti-mouse CD127 (IL-7Rα) Antibody
25microg
Catalog Number: 135017
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | IL-7Ra-IgG1 fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199. |
| Application References(PubMed link indicates BioLegend citation) | Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed |
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| Product Citations | Yamaguchi A, et al. 2021. Genes Cells. 26:782. PubMed Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed Kovacs SB, et al. 2020. Cell Reports. 32(4):107967. PubMed |
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| RRID | AB_1937206 (BioLegend Cat. No. 135017) AB_1937205 (BioLegend Cat. No. 135018) |
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| Structure | Type I transmembrane glycoprotein, associate with CD132, 60-90 kD |
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| Distribution | Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells |
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| Function | T cell and immature B cell proliferation and development |
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| Ligand/Receptor | IL-7 |
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| Cell Type | B cells, T cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. |
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| Gene ID | 16197 |
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| UniProt | View information about CD127 on UniProt.org |
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| Clone | A7R34 |
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| Regulatory Status | RUO |
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| Other Names | IL-7 receptor α chain, IL-7Rα |
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| Isotype | Rat IgG2a, κ |
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