Biolegend, 135117, Alexa Fluor® 488 anti-mouse CD117 (c-kit) Antibody
25microg
Catalog Number: 135117
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Murine IL-3 dependent mast cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3 ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
| Application References(PubMed link indicates BioLegend citation) | Yoshinaga K, et al. 1991. Development 113:689. Broudy VC, et al. 1996. Blood 88:75. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895. |
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| RRID | AB_2561667 (BioLegend Cat. No. 135118) |
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| Structure | Ig superfamily, 145 kD |
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| Distribution | Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells |
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| Function | Growth factor receptor, tyrosine kinase |
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| Ligand/Receptor | Stem Cell Factor (SCF) |
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| Cell Type | Embryonic Stem Cells, Mesenchymal Stem Cells |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ. et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P et al. 1986. Nature 320:415. 5. Witte ON. et al. 1990. Cell 63:5. |
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| Gene ID | 16590 |
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| UniProt | View information about CD117 on UniProt.org |
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| Clone | ACK2 |
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| Regulatory Status | RUO |
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| Other Names | c-KIT, Stem Cell Factor Receptor (SCFR) |
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| Isotype | Rat IgG2b, κ |
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