Biolegend, 135520, Alexa Fluor® 594 anti-mouse CD115 (CSF-1R) Antibody
100microg
Catalog Number: 135520
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154. |
| Application References(PubMed link indicates BioLegend citation) | Sudo T, et al. 1995. Oncogene 11:2469. Murayama T, et al. 1999. Circulation 99:1740. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed Dong L, et al. 2016. Nature. 539:304–308. PubMed |
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| Product Citations | Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed Kuboi Y, et al. 2019. Int Immunol. 31:287. PubMed Cuccarese M, et al. 2017. Nat Commun. 8:14293. PubMed Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed |
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| RRID | AB_2563315 (BioLegend Cat. No. 135520) |
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| Structure | Ig superfamily, 145 kD |
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| Distribution | Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts |
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| Function | Growth factor receptor, tyrosine kinase |
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| Ligand/Receptor | Macrophage colony stimulating factor (M-CSF), IL-34 |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Osteoclasts |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | Sudo T, et al. 1995 Oncogene 11:2469. Murayama T, et al. 1999 Circulation 99:1740. Goswami S, et al. 2005 Cancer Res. 65:5278. Yu W, et al. 2008 J. Leuko. Biol. 84:852. |
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| Gene ID | 12978 |
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| UniProt | View information about CD115 on UniProt.org |
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| Clone | AFS98 |
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| Regulatory Status | RUO |
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| Other Names | CSF-1R, M-CSFR, c-fms |
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| Isotype | Rat IgG2a, κ |
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