Biolegend, 135907, APC anti-mouse CD140a Antibody
25microg
Catalog Number: 135907
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse PDGFR-α-hIgG1 recombinant fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
| Application Notes | Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays. |
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| Application References(PubMed link indicates BioLegend citation) | Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F) |
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| Product Citations | Jadhav U et al. 2017. Cell stem cell. 21(1):65-77 . PubMed Korsunsky I, et al. 2022. Med. 3:481. PubMed Akasaka H, et al. 2023. JCI Insight. 8:. PubMed Wei Y, et al. 2021. Elife. 10: . PubMed Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed Agarwalla P, et al. 2022. Nat Biotechnol. 40:1250. PubMed Sastourné-Arrey Q, et al. 2023. Nat Commun. 14:80. PubMed Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed Wietecha MS, et al. 2020. Nat Commun. 2.266666667. PubMed Wang W, et al. 2019. Cell Metab. 30:174. PubMed Chen J, et al. 2021. Sci Adv. 7:. PubMed Vazquez-Armendariz AI, et al. 2021. STAR Protocols. 2(2):100594. PubMed Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed Zhang X, et al. 2020. Cell Reports. 28(3):792-803.e4.. PubMed Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed Chen YT, et al. 2021. Nat Commun. 12:6845. PubMed Ghelman J, et al. 2021. Glia. 69(11):2699-2716. PubMed Wang B,et al. 2017. Cell Discov.. 10.1038/celldisc.2017.36. PubMed Gui J, et al. 2020. Nat Cancer. 1:603. PubMed Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed Reddy A, et al. 2020. Cell. 183:62. PubMed Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Ko T, et al. 2022. Nat Commun. 13:3275. PubMed Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed Zhao T et al. 2018. Cell stem cell. 23(1):31-45 . PubMed Murakami Y, et al. 2019. Sci Rep. 1.188888889. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Jing Y, et al. 2020. Cancer Gene Ther. 27:910. PubMed Hogarth MW, et al. 2019. Nat Commun. 10:2430. PubMed Avraham S, et al. 2019. Oncogene. 38:3812. PubMed Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed Nowlan B, et al. 2019. Haematologica. 105:71. PubMed Hu Y, et al. 2021. EMBO Rep. 22:e52481. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Wang W, et al. 2014. Proc Natl Acad Sci U S A. 111:14466. PubMed Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed Moiseenko A, et al. 2020. Cell Rep. 33:108549. PubMed Lu Z, et al. 2021. J Clin Invest. 131:. PubMed Grainger N, et al. 2020. J Physiol. 598:3283. PubMed Cangkrama M, et al. 2020. EMBO Mol Med. 12:e11466. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Chandrakanthan V, et al. 2016. Proc Natl Acad Sci U S A. 113: 2306-2315. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Pinho AV, et al. 2018. Nat Commun. 9:5083. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed Wong PP, et al. 2020. Cell. 181(6):1346-1363.e21. PubMed |
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| RRID | AB_2043969 (BioLegend Cat. No. 135907) AB_2043970 (BioLegend Cat. No. 135908) |
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| Structure | Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding. |
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| Distribution | Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse. |
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| Function | Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils. |
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| Ligand/Receptor | PDGFs |
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| Cell Type | Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883 |
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| Gene ID | 18595 |
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| UniProt | View information about CD140a on UniProt.org |
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| Clone | APA5 |
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| Regulatory Status | RUO |
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| Other Names | PDGF receptor-α, PDGFR-α |
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| Isotype | Rat IgG2a, κ |
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