Biolegend, 137012, Alexa Fluor® 488 anti-mouse CD68 Antibody
100microg
Catalog Number: 137012
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Purified Con A receptor glycoproteins from the P815 cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested FC, 3D IHC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10. |
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| Application References(PubMed link indicates BioLegend citation) | Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB) Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC) Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC) Kayama H, et al. 2012. PNAS. 109:5010. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Barbour T, et al. 2016. Kidney Int. 89: 823-832. PubMed Mizutani T, et al. 2023. iScience. 26:106081. PubMed Fukuzaki Y, et al. 2023. iScience. 26:106340. PubMed Basta MD, et al. 2023. iScience. 26:106570. PubMed Zhou Y, et al. 2021. FASEB J. 35:e21288. PubMed Sun Z, et al. 2022. Br J Pharmacol. 179:1237. PubMed Chen O, et al. 2023. J Clin Invest. 133: . PubMed Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed Zhou Y, et al. 2019. Differentiation. 109:16:00. PubMed Wang LH, et al. 2021. Sci Adv. 7: . PubMed García L, et al. 2015. Exp Biol Med. 240: 904 - 916. PubMed DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed Paterson N, et al. 2022. Elife. 11:. PubMed Lv F, et al. 2022. iScience. 25:103639. PubMed Vegas A, et al. 2016. Nat Biotechnol. 10.1038/nbt.3462. PubMed Kawasaki N, et al. 2013. Proc Natl Acad Sci U S A. 110:7826. PubMed Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed DeDreu J, et al. 2022. FASEB J. 36:e21995. PubMed Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed |
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| RRID | AB_2074847 (BioLegend Cat. No. 137011) AB_2074846 (BioLegend Cat. No. 137012) |
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| Structure | A member of the lysosomal-associated membrane protein (lamp) family. |
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| Distribution | Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells. |
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| Function | Involved in cell-cell interaction or cell-ligand interaction, still not completely understood. |
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| Cell Type | Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Adhesion Molecules, CD Molecules, Innate Immune Signaling |
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| Antigen References | 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113. |
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| Gene ID | 12514 |
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| UniProt | View information about CD68 on UniProt.org |
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| Clone | FA-11 |
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| Regulatory Status | RUO |
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| Other Names | Macrosialin |
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| Isotype | Rat IgG2a |
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