Biolegend, 138005, Alexa Fluor® 647 anti-mouse CD144 (VE-cadherin) Antibody
25microg
Catalog Number: 138005
Loading…
| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | VE-cadherin-Ig fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope) |
|---|
| Product Citations | Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed Grünewald M, et al. 2021. Science. 373: . PubMed Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Himburg HA, et al. 2019. J Clin Invest. 130:315. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Rybtsov S, et al. 2011. J Exp Med. 208:1305. PubMed Kedem A, et al. 2017. J Ovarian Res. 10.1186/s13048-017-0354-z. PubMed Himburg H, et al. 2016. Nat Microbiol. 2:16232. PubMed Poulos M, et al. 2016. Nat Commun. 7:13829. PubMed Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed Zhang J, et al. 2021. Nature. 590:457. PubMed Wei Q, et al. 2020. Dev Cell. 53:503. PubMed Gordon-Keylock S, et al. 2013. Blood. 122:2338. PubMed Renner B, et al. 2013. J Am Soc Nephrol. 24:1849. PubMed Xu C, et al. 2018. Nat Commun. 9:2449. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Mizoguchi T, et al. 2014. Dev Cell. 29:340. PubMed Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed |
|---|
| RRID | AB_10568319 (BioLegend Cat. No. 138005) AB_10569114 (BioLegend Cat. No. 138006) |
|---|
| Structure | A 120 kD member of type II Cadherin family |
|---|
| Distribution | Endothelial cells |
|---|
| Function | Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion |
|---|
| Ligand/Receptor | CD144 |
|---|
| Cell Type | Endothelial cells, Mesenchymal Stem Cells |
|---|
| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology |
|---|
| Molecular Family | Adhesion Molecules, CD Molecules |
|---|
| Antigen References | 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. |
|---|
| Gene ID | 12562 |
|---|
| UniProt | View information about CD144 on UniProt.org |
|---|
| Clone | BV13 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Vascular endothelial-cadherin |
|---|
| Isotype | Rat IgG1, κ |
|---|
