Biolegend, 139321, Alexa Fluor® 647 anti-mouse CD64 (FcγRI) Antibody
25microg
Catalog Number: 139321
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | BALB/c mouse FcγRI-human IgG Fc fusion protein. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed Sierro F, et al. 2017. Immunity. 47:374. PubMed Ahmed A, et al. 2023. Mol Oncol. 1545:17. PubMed Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed Ferrer-Font L, et al. 2020. Elife. 9:. PubMed |
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| RRID | AB_2566560 (BioLegend Cat. No. 139321) AB_2566561 (BioLegend Cat. No. 139322) |
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| Structure | Ig superfamily, type I glycoprotein, 72 kD |
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| Distribution | Monocytes, macrophages, mast cells, dendritic cells |
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| Function | Phagocytosis, ADCC |
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| Ligand/Receptor | IgG |
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| Cell Type | Dendritic cells, Macrophages, Mast cells, Monocytes |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Gene ID | 14129 |
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| UniProt | View information about CD64 on UniProt.org |
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| Clone | X54-5/7.1 |
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| Regulatory Status | RUO |
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| Other Names | FcRI |
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| Isotype | Mouse IgG1, κ |
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