Biolegend, 146004, Alexa Fluor® 647 anti-Asialo-GM1 Antibody
100microg
Catalog Number: 146004
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| Verified Reactivity | Mouse, Rat |
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| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Immunogen | Asialo-GM1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2. This antibody can partially block IL-12 induced IFN-γ production but does not affect other systemic action of IL-12. This product may contain other non-IgG subtypes. |
| Application References(PubMed link indicates BioLegend citation) | Naiki M, et al. 1974. J. Immunol. 113:84. Kasai M, et al. 1981. Nature. 291:334. (Depletion) Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion) Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion) |
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| RRID | AB_2563195 (BioLegend Cat. No. 146003) AB_2563196 (BioLegend Cat. No. 146004) |
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| Structure | Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer |
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| Distribution | NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons |
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| Function | Supports cellular identification, neuronal plasticity, and repair mechanisms |
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| Interaction | Facilitates cellular recognition and cell-to-cell communication |
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| Ligand/Receptor | Ligand for cholera toxin |
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| Cell Type | Basophils, Neurons, NK cells, T cells |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Signal Transduction |
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| Antigen References | 1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 4. Bansal AS, et al. 1994. J. Clin. Path. 47:300. |
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| Gene ID | NA |
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| Clone | Poly21460 |
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| Regulatory Status | RUO |
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| Other Names | Ganglioside, GM1, ganglio-N-tetraosylceramide |
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| Isotype | Rabbit Polyclonal IgG |
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