Biolegend, 149523, Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) Antibody
25microg
Catalog Number: 149523
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | FCγR4 —EC domain fusion with IgG1 Fc |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin < EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team). |
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| Application References(PubMed link indicates BioLegend citation) | Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block) Nimmerjahn F, et al. 2005. Immunity 23:41. |
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| Product Citations | Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed |
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| RRID | AB_2687087 (BioLegend Cat. No. 149523) AB_2687088 (BioLegend Cat. No. 149524) |
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| Structure | 22.4 kD |
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| Distribution | Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine |
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| Function | Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory. |
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| Interaction | FCγR4 –EC domain fusion with IgG1 Fc |
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| Ligand/Receptor | IgG2a and IgG2b |
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| Cell Type | B cells, Leukocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | Mechetina LV, et al. 2002. Immunogenetics. 54:463-8. Nimmerjahn F, et al. 2005. Immunity. 23:41-51. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34. |
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| Gene ID | 246256 |
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| UniProt | View information about CD16.2 on UniProt.org |
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| Clone | 9E9 |
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| Regulatory Status | RUO |
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| Other Names | FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV |
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| Isotype | Armenian Hamster IgG |
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