Biolegend, 150431, Alexa Fluor® 488 anti-mouse CD20 Antibody
25microg
Catalog Number: 150431
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| Verified Reactivity | Mouse |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse CD20 - transfected cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| RRID |
|---|
| AB_2890699 (BioLegend Cat. No. 150431) AB_2890699 (BioLegend Cat. No. 150432) |
| Structure | Member of the MS4A family, four transmembrane spanning regions, three isoforms of 37, 35, and 37 kD, depending on the phosphorylation degree. |
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| Distribution | B cells and a subset of T cells. |
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| Function | Regulates B-cell activation and proliferation. |
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| Interaction | Associated with MHC class I and II, CD53, CD81, and CD82. |
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| Cell Type | B cells, T cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Morsy DE, et al. 2013. J. Immunol. 191:3112. 2. Lund FE, Randall TD. 2010. Nat. Rev. Immunol. 10:236. 3. Beers SA, et al. 2010. Blood 115:5191. 4. Kuijpers TW, et al. 2010. J. Clin. Invest. 120:214. |
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| Gene ID | 12482 |
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| UniProt | View information about CD20 on UniProt.org |
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| Clone | SA275A11 |
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| Regulatory Status | RUO |
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| Other Names | Ms4a1, Ly-44 |
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| Isotype | Rat IgG2b, κ |
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