Biolegend, 151204, Alexa Fluor® 488 anti-mouse/human Ki-67 Antibody
100microg
Catalog Number: 151204
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| Verified Reactivity | Mouse, Human |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested ICC, IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Product Citations | Chan S, et al. 2022. Nat Cancer. . PubMed Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed Wei Z, et al. 2021. Invest Ophthalmol Vis Sci. 62:29. PubMed Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed Huang FL, et al. 2022. Oncol Rep. 47:. PubMed Gao H, et al. 2021. Cells. 10:. PubMed Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed Lu J, et al. 2021. Cell Death Discov. 7:165. PubMed |
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| RRID | AB_2566800 (BioLegend Cat. No. 151204) |
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| Structure | 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats. |
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| Distribution | Nucleus and chromosomes. |
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| Function | Required for cell cycle progression and proliferation. |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication |
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| Antigen References | Starborg M, et al. 1996. J. Cell. Sci. 109:143. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294. |
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| Gene ID | 428817345 |
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| UniProt | View information about Ki-67 on UniProt.org |
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| Clone | 11F6 |
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| Regulatory Status | RUO |
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| Other Names | Mki67, Ki67, Ki-67, MIB-1, KIA |
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| Isotype | Rat IgG2b, κ |
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