Biolegend, 152205, Alexa Fluor® 647 anti-mouse CD34 Antibody
25microg
Catalog Number: 152205
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse CD34 transfected cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies. |
| Product Citations | Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed |
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| RRID | AB_2629649 (BioLegend Cat. No. 152205) |
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| Structure | Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD. |
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| Distribution | Hematopoietic progenitors, endothelial cells, brain and testis tissues. |
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| Function | Cell adhesion and homing. |
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| Interaction | PKC. |
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| Ligand/Receptor | L-selectin and E-selectin. |
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| Cell Type | Endothelial cells, Hematopoietic stem and progenitors |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77. 2. Brown J, et al. 1991. Int Immunol. 3:175. 3. Suda J, et al. 1992. Blood. 79:2288-95. 4. Baumhueter S, et al. 1994. Blood. 84:2554. |
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| Gene ID | 12490 |
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| UniProt | View information about CD34 on UniProt.org |
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| Clone | SA376A4 |
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| Regulatory Status | RUO |
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| Other Names | My10, gp105-120, mucosialin |
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| Isotype | Rat IgG2a, λ |
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