Biolegend, 154409, Alexa Fluor® 488 anti-mouse Perforin Antibody
25microg
Catalog Number: 154409
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | Mouse perforin transfected cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Clone S16009B reacts with perforin from both C57BL/6 and BALB/c strains. |
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| RRID | AB_3097554 (BioLegend Cat. No. 154409) AB_3097554 (BioLegend Cat. No. 154410) |
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| Structure | 70 kD Protein that oligomerizes for pore formation. |
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| Distribution | CTL, NK (Cytoplasmic Granules) |
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| Function | Target cell lysis |
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| Cell Type | NK cells, T cells |
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| Biology Area | Immunology |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | Podack ER, et al, 1988, Immunol Rev, 103:203 Konjar S, et al, 2010. Immunology, 131:257 Trapani JA, et al, 2002, Nat Rev Immunol, 2:735 Osinska I, et al. 2014, Cent Eur J Immnol, 39:109 |
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| Gene ID | 18646 |
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| UniProt | View information about Perforin on UniProt.org |
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| Clone | S16009B |
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| Regulatory Status | RUO |
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| Other Names | Prf1, Pfp, Pfn, Pfn-1 |
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| Isotype | Rat IgG2a, κ |
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