Biolegend, 201405, Alexa Fluor® 488 anti-rat CD3 Antibody
25microg
Catalog Number: 201405
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| Verified Reactivity | Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | F344 rat spleen cells stimulated with PMA and calcium ionophore |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2. |
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| Application References(PubMed link indicates BioLegend citation) | Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP) Nicholls MR, et al. 1993. Transplantation 55:459. (Block) Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF) Baba T, et al. 2006. Blood 107:2004. (IHC) Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P) Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed |
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| Product Citations | Besseling PJ, et al. 2023. APL Bioeng. 7:026107. PubMed Ferreira RC, et al. 2015. Diabetologia. 781:58. PubMed Wang Y, et al. 2021. Front Cell Dev Biol. 9:702890. PubMed |
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| RRID | AB_893303 (BioLegend Cat. No. 201406) |
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| Structure | Ig superfamily, approximately 20-25 kD |
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| Distribution | Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells |
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| Function | Antigen recognition, TCR signal transduction, T cell activation |
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| Ligand/Receptor | Peptide antigen/MHC complex |
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| Cell Type | NKT cells, T cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Tanaka T, et al. 1989 J. Immunol. 142:2791. 2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. |
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| Gene ID | 2571030067831560925300 |
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| UniProt | View information about CD3 on UniProt.org |
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| Clone | 1F4 |
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| Regulatory Status | RUO |
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| Other Names | T cell antigen receptor complex, T3 |
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| Isotype | Mouse IgM, κ |
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