Biolegend, 201406, Alexa Fluor® 488 anti-rat CD3 Antibody
100microg
Catalog Number: 201406
Send an RFQ.
Category
Loading…
| Verified Reactivity | Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | F344 rat spleen cells stimulated with PMA and calcium ionophore |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections1 and formaldehyde- fixed paraffin embedded sections4,5 immunofluorescence microscopy3, in vivo activation of T cell responses1, and in vivo inhibition of T cell responses2. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Tanaka T, et al. 1989. J. Immunol. 142:2791. (Activ, IHC, IP) Nicholls MR, et al. 1993. Transplantation 55:459. (Block) Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. (IF) Baba T, et al. 2006. Blood 107:2004. (IHC) Fujishiro J, et al. 2010. Am. J. Transplant. 10:1545-55. (IHC-P) Li X, et al. 2009. J. Immunol. 183:3955. (FC) PubMed |
|---|
| Product Citations | Besseling PJ, et al. 2023. APL Bioeng. 7:026107. PubMed Ferreira RC, et al. 2015. Diabetologia. 781:58. PubMed Wang Y, et al. 2021. Front Cell Dev Biol. 9:702890. PubMed |
|---|
| RRID | AB_893303 (BioLegend Cat. No. 201406) |
|---|
| Structure | Ig superfamily, approximately 20-25 kD |
|---|
| Distribution | Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells |
|---|
| Function | Antigen recognition, TCR signal transduction, T cell activation |
|---|
| Ligand/Receptor | Peptide antigen/MHC complex |
|---|
| Cell Type | NKT cells, T cells, Thymocytes |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | 1. Tanaka T, et al. 1989 J. Immunol. 142:2791. 2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74. |
|---|
| Gene ID | 2571030067831560925300 |
|---|
| UniProt | View information about CD3 on UniProt.org |
|---|
| Clone | 1F4 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | T cell antigen receptor complex, T3 |
|---|
| Isotype | Mouse IgM, κ |
|---|
