Biolegend, 201511, Alexa Fluor® 488 anti-rat CD4 Antibody
25microg
Catalog Number: 201511
Send an RFQ.
Category
Loading…
| Verified Reactivity | Rat |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Rat thymocyte membrane glycoproteins |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) Mannie MD, et al. 1993. J. Immunol. 151:7293. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed |
|---|
| Product Citations | Nuccitelli R, et al. 2015. PLoS One. 10: 0134364. PubMed Xiao L, et al. 2014. J Endod. 41:479. PubMed Qiu T, et al. 2022. Cancers (Basel). 14:. PubMed |
|---|
| RRID | AB_572038 (BioLegend Cat. No. 201511) |
|---|
| Structure | Ig superfamily, 55 kD |
|---|
| Distribution | Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells) |
|---|
| Function | T cell co-receptor, T cell activation |
|---|
| Ligand/Receptor | MHC class II molecule |
|---|
| Cell Type | Macrophages, T cells, Thymocytes |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules |
|---|
| Antigen References | 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649. |
|---|
| Gene ID | 24933 |
|---|
| UniProt | View information about CD4 on UniProt.org |
|---|
| Clone | W3/25 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | T4, L3T4, W3/25 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
