Biolegend, 201512, Alexa Fluor® 488 anti-rat CD4 Antibody
100microg
Catalog Number: 201512
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| Verified Reactivity | Rat |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Rat thymocyte membrane glycoproteins |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality testedIHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3. |
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| Application References(PubMed link indicates BioLegend citation) | Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) Shioji K, et al. 2001. Circulation Res. 89:540. (IHC) Mannie MD, et al. 1993. J. Immunol. 151:7293. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed |
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| Product Citations | Nuccitelli R, et al. 2015. PLoS One. 10: 0134364. PubMed Xiao L, et al. 2014. J Endod. 41:479. PubMed Qiu T, et al. 2022. Cancers (Basel). 14:. PubMed |
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| RRID | AB_572038 (BioLegend Cat. No. 201511) |
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| Structure | Ig superfamily, 55 kD |
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| Distribution | Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells) |
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| Function | T cell co-receptor, T cell activation |
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| Ligand/Receptor | MHC class II molecule |
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| Cell Type | Macrophages, T cells, Thymocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609. 2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649. |
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| Gene ID | 24933 |
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| UniProt | View information about CD4 on UniProt.org |
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| Clone | W3/25 |
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| Regulatory Status | RUO |
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| Other Names | T4, L3T4, W3/25 |
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| Isotype | Mouse IgG1, κ |
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