Biolegend, 260356, GMP APC/Fire™ 750 anti-human CD36 Antibody
100tests
Catalog Number: 260356
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| Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human platelets |
| Formulation | Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. |
| Concentration | 100 µg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunofluorescence2. |
| Application References(PubMed link indicates BioLegend citation) | Stelner E, et al. 2006. J. Cell Sci. 119:459. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF) |
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| Disclaimer | GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products |
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| Structure | Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD |
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| Distribution | Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells |
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| Function | Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion. |
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| Ligand/Receptor | Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V |
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| Cell Type | Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Hogg N, et al. 1984. Immunology 53-753. 2. Greenwalt DE, et al. 1992. Blood 80:1105. 3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985. 4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811. |
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| Gene ID | 948 |
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| UniProt | View information about CD36 on UniProt.org |
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| Clone | 5-271 |
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| Other Names | GPIIIb, GPIV |
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| Isotype | Mouse IgG2a, κ |
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