Biolegend, 285295, Spark Blue™ 550 anti-human CD11b (activated) (Flexi-Fluor™) Antibody
50microg
Catalog Number: 285295
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions. |
| Concentration | 0.2 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC |
| Recommended Usage | Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL. For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm. |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9. Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408). |
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| Additional Product Notes | For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line. |
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| Application References(PubMed link indicates BioLegend citation) | Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed Koleva RI, et al. 2012. Blood. 119:4878. PubMed. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed |
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| RRID | AB_3683225 (BioLegend Cat. No. 285295) |
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| Structure | Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD |
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| Distribution | Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset |
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| Function | Adhesion, phagocytosis, chemotaxis, neutrophil activation |
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| Ligand/Receptor | ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690. 2. Petty H, et al. 1996. Immunol. Today 17:209. |
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| Gene ID | 3684 |
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| UniProt | View information about CD11b on UniProt.org |
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| Clone | CBRM1/5 |
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| Regulatory Status | RUO |
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| Other Names | Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM |
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| Isotype | Mouse IgG1, κ |
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