Biolegend, 300421, Alexa Fluor® 488 anti-human CD3 Antibody
25tests
Catalog Number: 300421
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICC, IHC-F - Verified |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume For flow cytometric staining using the test size, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). |
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| Application References(PubMed link indicates BioLegend citation) | Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Hu X, et al. 2023. Nat Biotechnol. . PubMed Gilman KE, et al. 2023. Front Immunol. 13:1045710. PubMed Tolcher AW, et al. 2023. J Immunother Cancer. 11: . PubMed Li C, et al. 2022. J Exp Med. 219:. PubMed Junker F, et al. 2021. Cytometry A. 99:832. PubMed Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed Kim D, et al. 2020. Nat Med. 26:236. PubMed Riding AM, et al. 2022. iScience. 25:104660. PubMed VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed Marquardsen F, et al. 2017. J Clin Immunol. 10.1007/s10875-017-0431-5. PubMed Burel JG, et al. 2019. Elife. 8. PubMed Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed Riese P, et al. 2022. Nat Commun. 13:6894. PubMed Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed |
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| RRID | AB_2564149 (BioLegend Cat. No. 300454) AB_389310 (BioLegend Cat. No. 300415) |
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| Structure | Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD |
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| Distribution | Mature T and NK T cells, thymocyte differentiation |
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| Function | Antigen recognition, signal transduction, T cell activation |
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| Ligand/Receptor | Peptide antigen bound to MHC |
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| Cell Type | NKT cells, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, TCRs |
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| Antigen References | 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. |
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| Gene ID | 916 |
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| UniProt | View information about CD3 on UniProt.org |
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| Clone | UCHT1 |
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| Regulatory Status | RUO |
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| Workshop | III 471 |
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| Other Names | T3, CD3ε |
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| Isotype | Mouse IgG1, κ |
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