Biolegend, 300422, Alexa Fluor® 647 anti-human CD3 Antibody
25tests
Catalog Number: 300422
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested ICC, IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). |
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| Application References(PubMed link indicates BioLegend citation) | Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Mascarau R, et al. 2023. J Cell Biol. 222:. PubMed Meehan S, et al. 2019. Commun Biol. 2:229. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Shilts J, et al. 2022. Nature. 608:397. PubMed Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed Chen K, et al. 2012. PLoS One. 7:e39294. PubMed Han G, et al. 2018. Nat Protoc. 2.014583333. PubMed Wegrecki M, et al. 2022. Nat Commun. 13:3872. PubMed Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed |
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| RRID | AB_493092 (BioLegend Cat. No. 300422) AB_389332 (BioLegend Cat. No. 300416) |
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| Structure | Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD |
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| Distribution | Mature T and NK T cells, thymocyte differentiation |
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| Function | Antigen recognition, signal transduction, T cell activation |
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| Ligand/Receptor | Peptide antigen bound to MHC |
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| Cell Type | NKT cells, T cells, Thymocytes, Tregs |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, TCRs |
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| Antigen References | 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. |
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| Gene ID | 916 |
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| UniProt | View information about CD3 on UniProt.org |
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| Clone | UCHT1 |
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| Regulatory Status | RUO |
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| Workshop | III 471 |
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| Other Names | T3, CD3ε |
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| Isotype | Mouse IgG1, κ |
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