Biolegend, 300616, Alexa Fluor® 647 anti-human CD5 Antibody
100tests
Catalog Number: 300616
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5. |
| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC) Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC) |
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| Product Citations | Hui HYL, et al. 2021. Curr Protoc. 1:e260. PubMed |
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| RRID | AB_493037 (BioLegend Cat. No. 300616) |
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| Structure | Scavenger receptor superfamily, 67 kD |
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| Distribution | T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells |
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| Function | Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction |
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| Ligand/Receptor | CD72 |
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| Cell Type | B cells, Dendritic cells, Leukemia, T cells, Thymocytes |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Kipps T. 1988. Adv. Immunol. 47:117. 2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5. 3. Wood GS, et al. 1992. Am. J. Pathol. 14:789. |
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| Gene ID | 921 |
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| UniProt | View information about CD5 on UniProt.org |
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| Clone | UCHT2 |
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| Regulatory Status | RUO |
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| Workshop | III 518 |
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| Other Names | Leu-1, Ly-1, T1, Tp67 |
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| Isotype | Mouse IgG1, κ |
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