Biolegend, 301414, Alexa Fluor® 647 anti-human CD11b (activated) Antibody
100tests
Catalog Number: 301414
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Red Laser (633 nm) |
| Application Notes | The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the a chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9. Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408). |
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| Application References(PubMed link indicates BioLegend citation) | Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed Koleva RI, et al. 2012. Blood. 119:4878. PubMed. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed |
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| RRID | AB_2715797 (BioLegend Cat. No. 301413) AB_2715798 (BioLegend Cat. No. 301414) |
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| Structure | Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD |
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| Distribution | Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset |
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| Function | Adhesion, phagocytosis, chemotaxis, neutrophil activation |
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| Ligand/Receptor | ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X |
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| Cell Type | B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690. 2. Petty H, et al. 1996. Immunol. Today 17:209. |
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| Gene ID | 3684 |
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| UniProt | View information about CD11b on UniProt.org |
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| Clone | CBRM1/5 |
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| Regulatory Status | RUO |
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| Other Names | Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM |
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| Isotype | Mouse IgG1, κ |
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