Biolegend, 301622, Alexa Fluor® 647 anti-human CD11c Antibody
100microg
Catalog Number: 301622
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Chimpanzee, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | µg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For µg size, the suggested use of this reagent for immunofluorescent staining is ≤2 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC) Ottonello L, et al. 1999. Blood 93:3505. Metelitsa LS, et al. 2002. Blood 99:4166. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed Ihanus E, et al. 2007. Blood 109:802-810. Gurer C, et al. 2008. Blood 112:1231. PubMed Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC) |
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| Product Citations | Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed Toriyama M, et al. 2023. Front Mol Biosci. 10:1149828. PubMed Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed Sándor N, et al. 2013. Immunobiology. 218:652. PubMed Bristol JA, et al. 2022. PLoS Pathog. 18:e1010453. PubMed Paulson K, et al. 2010. Circ Res. 106:383. PubMed Sprokholt J,et al. 2017. PLoS One. 10.1371/journal.pone.0185580. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed |
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| RRID | AB_439792 (BioLegend Cat. No. 301619) AB_1088990 (BioLegend Cat. No. 301622) AB_439793 (BioLegend Cat. No. 301620) |
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| Structure | Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD |
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| Distribution | Myeloid, dendritic cells, NK cells, B cells and T cell subsets |
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| Function | Adhesion, CTL killing |
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| Ligand/Receptor | CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 |
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| Cell Type | B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810. |
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| Gene ID | 3687 |
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| UniProt | View information about CD11c on UniProt.org |
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| Clone | 3.9 |
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| Regulatory Status | RUO |
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| Workshop | III NL707 |
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| Other Names | Integrin αX subunit, CR4, p150, ITGAX |
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| Isotype | Mouse IgG1, κ |
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