Biolegend, 301639, Purified anti-human CD11c (Maxpar® Ready) Antibody
100microg
Catalog Number: 301639
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Chimpanzee, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
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| Application Notes |
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| Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC) Ottonello L, et al. 1999. Blood 93:3505. Metelitsa LS, et al. 2002. Blood 99:4166. Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed Ihanus E, et al. 2007. Blood 109:802-810. Gurer C, et al. 2008. Blood 112:1231. PubMed Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC) |
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| Product Citations | Van Tilbeurgh M, et al. 2022. Cell Rep Med. 3:100751. PubMed Stensland ZC, et al. 2022. iScience. 25:103626. PubMed |
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| RRID | AB_2562812 (BioLegend Cat. No. 301639) |
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| Structure | Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD |
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| Distribution | Myeloid, dendritic cells, NK cells, B cells and T cell subsets |
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| Function | Adhesion, CTL killing |
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| Ligand/Receptor | CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 |
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| Cell Type | B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810. |
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| Gene ID | 3687 |
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| UniProt | View information about CD11c on UniProt.org |
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| Clone | 3.9 |
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| Regulatory Status | RUO |
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| Workshop | III NL707 |
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| Other Names | Integrin αX subunit, CR4, p150, ITGAX |
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| Isotype | Mouse IgG1, κ |
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