Biolegend, 301717, Purified anti-human CD13 (Maxpar® Ready) Antibody
100microg
Catalog Number: 301717
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| Verified Reactivity | Human |
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| Reported Reactivity | Baboon, Chimpanzee, Cotton-topped Tamarin |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg). |
| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Saiki I, et al. 1993. Int J Cancer. 54:137. (Block) Rosenzwajg M, et al. 2000. Blood 95:453. (Block) Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC) |
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| RRID | AB_2563717 (BioLegend Cat. No. 301717) |
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| Structure | Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD |
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| Distribution | Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset |
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| Function | Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine |
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| Ligand/Receptor | Coronavirus receptor |
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| Cell Type | Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils |
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| Biology Area | Immunology, Stem Cells |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Shipp M, et al. 1993. Blood 82:1052. 2. Larsen S, et al. 1996. J. Exp. Med. 184:183. |
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| Gene ID | 290 |
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| UniProt | View information about CD13 on UniProt.org |
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| Clone | WM15 |
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| Regulatory Status | RUO |
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| Workshop | IV M44 |
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| Other Names | Aminopeptidase N, APN, gp150, ANPEP |
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| Isotype | Mouse IgG1, κ |
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