Biolegend, 302020, Alexa Fluor® 647 anti-human CD16 Antibody
100tests
Catalog Number: 302020
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human PMN cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058). |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) Hoshino S, et al. 1991. Blood 78:3232. (Stim) Tamm A, et al. 1996. Immunol. 157:1576. (Block) Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) Holl V, et al. 2004. J. Immunol. 173:6274. (Block) Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rout N, et al. 2010. PLoS One 5:e9787. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed Wu Z, et al. 2013. J. Virol. 87:7717. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim) |
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| Product Citations | Cansever D, et al. 2023. Nat Immunol. . PubMed Xu C, et al. 2022. iScience. 25:105123. PubMed Meehan S, et al. 2019. Commun Biol. 2:229. PubMed Zhichao Fan et al. 2019. Cell reports. 26(1):119-130 . PubMed Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed |
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| RRID | AB_492977 (BioLegend Cat. No. 302023) AB_492976 (BioLegend Cat. No. 302020) |
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| Structure | Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD) |
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| Distribution | NK cells, activated monocytes, macrophages, neutrophils |
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| Function | Low affinity IgG Fc receptor, phagocytosis, ADCC |
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| Ligand/Receptor | Aggregated IgG, IgG-antigen complex |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381. |
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| Gene ID | 2214 |
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| UniProt | View information about CD16 on UniProt.org |
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| Clone | 3G8 |
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| Regulatory Status | RUO |
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| Workshop | V NK80 |
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| Other Names | FcγRIII, Fc gamma receptor, Fc gamma receptor 3 |
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| Isotype | Mouse IgG1, κ |
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