Biolegend, 302051, Purified anti-human CD16 (Maxpar® Ready) Antibody
100microg
Catalog Number: 302051
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human PMN cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified PG - Reported in the literature, not verified in house |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
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| Application Notes |
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| The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058). |
| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
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| Application References(PubMed link indicates BioLegend citation) | Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP) Hoshino S, et al. 1991. Blood 78:3232. (Stim) Tamm A, et al. 1996. Immunol. 157:1576. (Block) Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC) Holl V, et al. 2004. J. Immunol. 173:6274. (Block) Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block) Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Rout N, et al. 2010. PLoS One 5:e9787. (FC) Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC) Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed Wu Z, et al. 2013. J. Virol. 87:7717. PubMed Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG) Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim) |
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| Product Citations | McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed Fenton TM, et al. 2020. Immunity. 52(3):557-570. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed |
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| RRID | AB_2562814 (BioLegend Cat. No. 302051) |
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| Structure | Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD) |
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| Distribution | NK cells, activated monocytes, macrophages, neutrophils |
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| Function | Low affinity IgG Fc receptor, phagocytosis, ADCC |
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| Ligand/Receptor | Aggregated IgG, IgG-antigen complex |
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| Cell Type | Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275. 2. Stroncek D, et al. 1991. Blood 77:1572. 3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381. |
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| Gene ID | 2214 |
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| UniProt | View information about CD16 on UniProt.org |
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| Clone | 3G8 |
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| Regulatory Status | RUO |
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| Workshop | V NK80 |
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| Other Names | FcγRIII, Fc gamma receptor, Fc gamma receptor 3 |
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| Isotype | Mouse IgG1, κ |
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