Biolegend, 302316, Alexa Fluor® 488 anti-human CD20 Antibody
100tests
Catalog Number: 302316
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human tonsillar B cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Polyak MJ, et al. 2002. Blood 99:3256. (IP) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) |
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| Product Citations | Lindner JM, et al. 2019. Immunity. 50:668. PubMed Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed Ferrari M, et al. 2021. J Virol. 95:e0068521. PubMed Pérol L, et al. 2016. Nat Commun. 7:13027. PubMed Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed |
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| RRID | AB_493227 (BioLegend Cat. No. 302316) |
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| Structure | Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD |
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| Distribution | B cell, T cell subsets |
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| Function | B cell activation |
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| Ligand/Receptor | Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82 |
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| Cell Type | B cells, T cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450. |
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| Gene ID | 931 |
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| UniProt | View information about CD20 on UniProt.org |
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| Clone | 2H7 |
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| Regulatory Status | RUO |
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| Workshop | IV B201 |
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| Other Names | B1, Bp35 |
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| Isotype | Mouse IgG2b, κ |
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