Biolegend, 302343, Purified anti-human CD20 (Maxpar® Ready) Antibody
100microg
Catalog Number: 302343
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human tonsillar B cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
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| Application Notes |
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| The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5. |
| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Polyak MJ, et al. 2002. Blood 99:3256. (IP) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) |
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| Product Citations | Fox-Fisher I, et al. 2022. Med (N Y). 3:468. PubMed Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed van Montfoort N, et al. 2018. Cell. 175:1744. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Hwang B, et al. 2021. Nat Methods. 18:903. PubMed |
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| RRID | AB_2562816 (BioLegend Cat. No. 302343) |
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| Structure | Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD |
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| Distribution | B cell, T cell subsets |
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| Function | B cell activation |
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| Ligand/Receptor | Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82 |
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| Cell Type | B cells, T cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450. |
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| Gene ID | 931 |
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| UniProt | View information about CD20 on UniProt.org |
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| Clone | 2H7 |
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| Regulatory Status | RUO |
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| Workshop | IV B201 |
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| Other Names | B1, Bp35 |
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| Isotype | Mouse IgG2b, κ |
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