Biolegend, 302616, Alexa Fluor® 488 anti-human CD25 Antibody
100tests
Catalog Number: 302616
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| Verified Reactivity | Human |
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| Reported Reactivity | Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Additional reported applications include: immunocytochemistry3. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed |
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| Product Citations | Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed Le Gall C, et al. 2022. J Immunother Cancer. 10:. PubMed Angiari S, et al. 2020. Cell Metab. 31:391. PubMed Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed Baratella M, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005285. PubMed Pais H, et al. 2019. Sci Rep. 9:5760. PubMed |
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| RRID | AB_493044 (BioLegend Cat. No. 302615) AB_493043 (BioLegend Cat. No. 302616) |
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| Structure | Type I transmembrane glycoprotein, 55 kD |
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| Distribution | Activated T cells and B cells, monocytes/macrophages, Treg |
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| Function | Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex |
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| Ligand/Receptor | IL-2 |
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| Cell Type | B cells, Macrophages, Monocytes, T cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681. |
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| Gene ID | 3559 |
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| UniProt | View information about CD25 on UniProt.org |
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| Clone | BC96 |
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| Regulatory Status | RUO |
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| Workshop | V T-072 |
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| Other Names | Low affinity IL-2R, IL-2R α chain, Tac, p55 |
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| Isotype | Mouse IgG1, κ |
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