Biolegend, 302617, Alexa Fluor® 647 anti-human CD25 Antibody
25tests
Catalog Number: 302617
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| Verified Reactivity | Human |
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| Reported Reactivity | Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications include: immunocytochemistry3. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed |
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| Product Citations | Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Tiittanen M, et al. 2013. PLoS One. 7:78420. PubMed Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed Singh KS, et al. 2021. Nature. 589:597. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed |
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| RRID | AB_493046 (BioLegend Cat. No. 302617) AB_493045 (BioLegend Cat. No. 302618) |
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| Structure | Type I transmembrane glycoprotein, 55 kD |
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| Distribution | Activated T cells and B cells, monocytes/macrophages, Treg |
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| Function | Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex |
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| Ligand/Receptor | IL-2 |
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| Cell Type | B cells, Macrophages, Monocytes, T cells, Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681. |
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| Gene ID | 3559 |
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| UniProt | View information about CD25 on UniProt.org |
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| Clone | BC96 |
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| Regulatory Status | RUO |
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| Workshop | V T-072 |
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| Other Names | Low affinity IL-2R, IL-2R α chain, Tac, p55 |
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| Isotype | Mouse IgG1, κ |
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