Biolegend, 303112, Alexa Fluor® 647 anti-human CD31 Antibody
100tests
Catalog Number: 303112
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Category
| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Jeon NL, et al. 2020. Biotechnol Bioeng. 117:748. PubMed Al-Akashi Z, et al. 2023. Front Cell Dev Biol. 11:1089945. PubMed Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed Sugahara K, et al. 2015. J Control Release. 212: 59-69. PubMed Shin N, et al. 2022. Biotechnol Bioeng. 119:566. PubMed Mendes R, et al. 2016. Infect Immun . 84: 2141 - 2148. PubMed Aplin J, et al. 2015. Placenta. Available online 11 August 2015. PubMed Kim J, et al. 2015. PLoS One. 10: 0133880. PubMed He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed Khoo A, et al. 2012. PLoS One. 7:e29250. PubMed Jühling F, et al. 2021. Life Sci Alliance. 4: . PubMed |
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| RRID | AB_493077 (BioLegend Cat. No. 303111) AB_493076 (BioLegend Cat. No. 303112) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 130-140 kD |
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| Distribution | Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset |
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| Function | Cell adhesion, signal transduction |
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| Ligand/Receptor | CD38 |
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| Cell Type | Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets |
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| Biology Area | Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229. |
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| Gene ID | 5175 |
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| UniProt | View information about CD31 on UniProt.org |
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| Clone | WM59 |
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| Regulatory Status | RUO |
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| Workshop | V P025 |
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| Other Names | PECAM-1, EndoCAM |
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| Isotype | Mouse IgG1, κ |
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