Biolegend, 303127, Purified anti-human CD31 (Maxpar® Ready) Antibody
100microg
Catalog Number: 303127
Send an RFQ.
Category
| Verified Reactivity | Human, Cynomolgus, Rhesus |
|---|---|
| Reported Reactivity | African Green, Baboon |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 1.0 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality tested CyTOF® - Verified |
| Recommended Usage | This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody. |
| Additional Product Notes | Maxpar® is a registered trademark of Standard BioTools Inc. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF) Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block) Nagano M, et al. 2007. Blood 110:151. (FC) PubMed MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
|---|
| Product Citations | Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed |
|---|
| RRID | AB_2563740 (BioLegend Cat. No. 303127) |
|---|
| Structure | Ig superfamily, type I transmembrane glycoprotein, 130-140 kD |
|---|
| Distribution | Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset |
|---|
| Function | Cell adhesion, signal transduction |
|---|
| Ligand/Receptor | CD38 |
|---|
| Cell Type | Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets |
|---|
| Biology Area | Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience |
|---|
| Molecular Family | Adhesion Molecules, CD Molecules |
|---|
| Antigen References | DeLisser H, et al. 1994. Immunol. Today 15:490. Newman P, 1997. J. Clin. Invest. 99:3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229. |
|---|
| Gene ID | 5175 |
|---|
| UniProt | View information about CD31 on UniProt.org |
|---|
| Clone | WM59 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | V P025 |
|---|
| Other Names | PECAM-1, EndoCAM |
|---|
| Isotype | Mouse IgG1, κ |
|---|