Biolegend, 304612, Alexa Fluor® 647 anti-human CD56 (NCAM) Antibody
100tests
Catalog Number: 304612
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| Verified Reactivity | Human |
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| Reported Reactivity | Cow, Pig |
| Antibody Type | Monoclonal |
| Host Species | Human |
| Immunogen | KG-1 human acute myelogenous leukemia cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing). |
| Application References(PubMed link indicates BioLegend citation) | Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. |
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| RRID | AB_493181 (BioLegend Cat. No. 304612) |
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| Structure | Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD |
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| Distribution | NK cells, T subset, neural tissue, some LGL and myeloid leukemias |
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| Function | Adhesion |
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| Ligand/Receptor | Heparin sulfate |
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| Cell Type | Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Lanier L, et al. 1991. J. Immunol. 146:4421. 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71. 3. Cremer H, et al. 1994. Nature 367:455. |
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| Gene ID | 4684 |
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| UniProt | View information about CD56 on UniProt.org |
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| Clone | MEM-188 |
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| Regulatory Status | RUO |
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| Workshop | VI NK26 |
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| Other Names | NCAM, Leu-19, NKH1 |
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| Isotype | Mouse IgG2a, κ |
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