Biolegend, 304817, Alexa Fluor® 647 anti-human CD62L Antibody
25tests
Catalog Number: 304817
Send an RFQ.
Category
Pack Size62 L
Loading…
| Verified Reactivity | Human |
|---|---|
| Reported Reactivity | Chimpanzee, Cow |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Concentrated supernatant from PMA-activated human peripheral blood leukocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858). |
|---|
| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed |
|---|
| Product Citations | Mauracher LM, et al. 2023. Res Pract Thromb Haemost. 7:100126. PubMed Santos-Gomes G, et al. 2014. Vaccine. 32:1247. PubMed Jung Y, et al. 2016. Proc Natl Acad Sci U S A. 113: E5916 - E5924. PubMed Zhou L, et al. 2012. Clin Vaccine Immunol. 19:1065. PubMed |
|---|
| RRID | AB_10719092 (BioLegend Cat. No. 304818) |
|---|
| Structure | Selectin, single chain glycoprotein, 74-95 kD |
|---|
| Distribution | Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes |
|---|
| Function | Leukocyte homing, leukocyte tethering, rolling |
|---|
| Ligand/Receptor | CD34, GlyCAM, MAdCAM-1 |
|---|
| Cell Type | B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs |
|---|
| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity |
|---|
| Molecular Family | Adhesion Molecules, CD Molecules |
|---|
| Antigen References | Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805. |
|---|
| Gene ID | 6402 |
|---|
| UniProt | View information about CD62L on UniProt.org |
|---|
| Clone | DREG-56 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | V S056 |
|---|
| Other Names | L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1 |
|---|
| Isotype | Mouse IgG1, κ |
|---|
