Biolegend, 304818, Alexa Fluor® 647 anti-human CD62L Antibody
100tests
Catalog Number: 304818
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Category
Pack Size62 L
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee, Cow |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Concentrated supernatant from PMA-activated human peripheral blood leukocytes |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858). |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed |
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| Product Citations | Mauracher LM, et al. 2023. Res Pract Thromb Haemost. 7:100126. PubMed Santos-Gomes G, et al. 2014. Vaccine. 32:1247. PubMed Jung Y, et al. 2016. Proc Natl Acad Sci U S A. 113: E5916 - E5924. PubMed Zhou L, et al. 2012. Clin Vaccine Immunol. 19:1065. PubMed |
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| RRID | AB_10719092 (BioLegend Cat. No. 304818) |
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| Structure | Selectin, single chain glycoprotein, 74-95 kD |
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| Distribution | Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes |
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| Function | Leukocyte homing, leukocyte tethering, rolling |
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| Ligand/Receptor | CD34, GlyCAM, MAdCAM-1 |
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| Cell Type | B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs |
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| Biology Area | Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805. |
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| Gene ID | 6402 |
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| UniProt | View information about CD62L on UniProt.org |
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| Clone | DREG-56 |
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| Regulatory Status | RUO |
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| Workshop | V S056 |
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| Other Names | L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1 |
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| Isotype | Mouse IgG1, κ |
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