Biolegend, 304917, Alexa Fluor® 647 anti-human CD62P (P-Selectin) Antibody
25tests
Catalog Number: 304917
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948). |
| Application References(PubMed link indicates BioLegend citation) | Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Yen YT, et al. 2006. J. Virol. 80:2684. Sato Y, et al. 2005. Blood 106:428. (IHC) |
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| Product Citations | Loelius SG et al. 2018. Current protocols in toxicology. 76(1):e46 . PubMed Cruz MA, et al. 2022. Nat Nanotechnol. 17:1004. PubMed Modery-Pawlowski C, et al. 2013. Biomaterials. 34:3031. PubMed Verheul H, et al. 2007. Clin Cancer Res. 4.250694444. PubMed Jooss NJ, et al. 2019. Int J Mol Sci. 20. PubMed Huang J, et al. 2022. Int J Mol Sci. 23:. PubMed Jahn K, et al. 2020. Blood Adv. 4:6315. PubMed Fernández DI, et al. 2022. iScience. 25:103718. PubMed Hrdinova J, et al. 2022. Int J Mol Sci. 23:. PubMed Sachs L, et al. 2022. Commun Biol. 5:86. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed |
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| RRID | AB_2185110 (BioLegend Cat. No. 304918) |
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| Structure | Selectin, type I glycoprotein, 140 kD |
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| Distribution | Activated platelets, megakaryocytes, endothelial cells |
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| Function | Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions |
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| Ligand/Receptor | CD162 (PSGL-1), CD24 and sialylated Lewis X |
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| Cell Type | Endothelial cells, Megakaryocytes, Neutrophils, Platelets |
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| Biology Area | Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025. 2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390. |
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| Gene ID | 6403 |
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| UniProt | View information about CD62P on UniProt.org |
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| Clone | AK4 |
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| Regulatory Status | RUO |
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| Workshop | VI P-44 |
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| Other Names | GMP-140, PADGEM |
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| Isotype | Mouse IgG1, κ |
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