Biolegend, 305012, Alexa Fluor® 647 anti-human CD64 Antibody
100tests
Catalog Number: 305012
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections12. |
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| Application References(PubMed link indicates BioLegend citation) | McMichael A, et al. Eds. 1987. Leucocyte Typing III. Oxford University Press. New York. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. p. 874. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Holl V, et al. 2004. J. Immunol. 173:6274. Hober D, et al. 2002. J. Gen. Virol. 83:2169. Cho HJ, et al. 2007. Physiol Genomics 149:60. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717. PubMed Bruhns P, et al. 2008. Blood 113:3716. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Carter DL, et al. 1999. Cytometry 37:41. (FC) Dougherty GJ, et al. 1987. Eur. J. Immunol. 17:1453. Blom AB, et al. 2003. Arthritis Rheum. 48(4):1002-14. (IHC) |
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| Product Citations | Poel C, et al. 2010. Blood. 116:5327. PubMed Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed Bassil R, et al. 2021. Nat Commun. 12:5220. PubMed |
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| RRID | AB_528867 (BioLegend Cat. No. 305012) |
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| Structure | Ig superfamily, type I glycoprotein, 72 kD |
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| Distribution | Monocytes, macrophages, dendritic cells, activated granulocytes |
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| Function | Phagocytosis, ADCC |
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| Ligand/Receptor | IgG receptor |
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| Cell Type | Dendritic cells, Granulocytes, Macrophages, Monocytes |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Fc Receptors |
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| Antigen References | 1. Hulett M, et al. 1994. Adv. Immunol. 57:1. 2. van de Winkel J, et al. 1993. Immunol. Today 14:215. |
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| Gene ID | 2209 |
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| UniProt | View information about CD64 on UniProt.org |
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| Clone | 10.1 |
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| Regulatory Status | RUO |
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| Workshop | VI MA36 |
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| Other Names | FcγRI, FcR I, FCGR1A |
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| Isotype | Mouse IgG1, κ |
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