Biolegend, 305216, Alexa Fluor® 647 anti-human CD80 Antibody
100tests
Catalog Number: 305216
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| Verified Reactivity | Human |
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| Reported Reactivity | Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246). |
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| Application References(PubMed link indicates BioLegend citation) | Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed |
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| Product Citations | Haile S, et al. 2013. J Immunol. 191:2829. PubMed Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed Wu Y, et al. 2022. J Cancer Res Clin Oncol. . PubMed Navarro-Guerrero E, et al. 2021. Sci Rep. 11:4245. PubMed |
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| RRID | AB_528874 (BioLegend Cat. No. 305215) AB_528875 (BioLegend Cat. No. 305216) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 60 kD |
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| Distribution | Activated B cells and T cells, macrophages, and dendritic cells |
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| Function | T cell costimulation |
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| Ligand/Receptor | CD28, CD152 (CTLA-4) |
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| Cell Type | B cells, Dendritic cells, Macrophages, T cells, Tregs |
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| Biology Area | Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers |
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| Molecular Family | CD Molecules, Immune Checkpoint Receptors |
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| Antigen References | 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561. |
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| Gene ID | 941 |
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| UniProt | View information about CD80 on UniProt.org |
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| Clone | 2D10 |
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| Regulatory Status | RUO |
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| Workshop | VI CD80.1 |
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| Other Names | B7-1, B7, BB1 |
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| Isotype | Mouse IgG1, κ |
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