Biolegend, 305511, Alexa Fluor® 647 anti-human CD94 Antibody
25tests
Catalog Number: 305511
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Category
| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | NK cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7. |
| Application References(PubMed link indicates BioLegend citation) | Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54. Phillip J, et al. 1996. Immunity 5:163. Lazetic S, et al. 1996. J. Immunol. 157:4741. Lanier LL, et al. 1998. Immunity 8:693. Wooden SL, et al. 2005. J. Immunol. 175:1383. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| RRID | AB_2249691 (BioLegend Cat. No. 305512) |
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| Structure | C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD |
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| Distribution | NK cells , T subset |
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| Function | Inhibits NK function |
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| Cell Type | NK cells, T cells |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165. 2. Moretta A, et al. 1997. Immunol. Rev. 155:105. |
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| Gene ID | 3824 |
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| UniProt | View information about CD94 on UniProt.org |
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| Clone | DX22 |
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| Regulatory Status | RUO |
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| Other Names | KP43 |
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| Isotype | Mouse IgG1, κ |
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