Biolegend, 305618, Alexa Fluor® 647 anti-human CD95 (Fas) Antibody
100tests
Catalog Number: 305618
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | CD95 transfected L cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656). Note: EOS9.1 antibody can induce apoptosis without cross-linking. |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop) Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC) Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC) Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Rout N, et al. 2010. PLoS One 5:e9787. (FC) Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed |
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| Product Citations | Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed Smulski C, et al. 2017. Cell Death Dis. 8:e2601. PubMed |
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| RRID | AB_528893 (BioLegend Cat. No. 305618) |
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| Structure | TNFR superfamily, type I transmembrane protein, 45 kD |
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| Distribution | T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes |
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| Function | Mediates apoptosis |
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| Ligand/Receptor | Fas ligand (CD178) |
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| Cell Type | B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Krammer P, et al. 1994. Immunol. Rev. 142:175. 2. Nagata S, et al. 1995. Science 267:1449. |
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| Gene ID | 355 |
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| UniProt | View information about CD95 on UniProt.org |
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| Clone | DX2 |
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| Regulatory Status | RUO |
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| Workshop | VI C-64 |
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| Other Names | Fas, APO-1, TNFRSF6 |
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| Isotype | Mouse IgG1, κ |
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