Biolegend, 306023, Alexa Fluor® 647 anti-human CD123 Antibody
25tests
Catalog Number: 306023
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| Verified Reactivity | Human |
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| Reported Reactivity | Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human IL-3Rα transfected COS cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7. |
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| Application References(PubMed link indicates BioLegend citation) | Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC) |
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| Product Citations | Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed Huang P, et al. 2020. Proc Natl Acad Sci U S A. 117:12868. PubMed |
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| RRID | AB_2562069 (BioLegend Cat. No. 306023) AB_2562070 (BioLegend Cat. No. 306024) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD |
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| Distribution | Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes |
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| Function | Hematopoietic cell proliferation, differentiation |
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| Ligand/Receptor | IL-3 |
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| Cell Type | Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Miyajima A, et al. 1993. Blood 82:1960. |
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| Gene ID | 3563 |
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| UniProt | View information about CD123 on UniProt.org |
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| Clone | 6H6 |
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| Regulatory Status | RUO |
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| Other Names | IL-3Rα, IL-3 Receptor alpha |
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| Isotype | Mouse IgG1, κ |
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