Biolegend, 306035, Alexa Fluor® 488 anti-human CD123 Antibody
25tests
Catalog Number: 306035
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Reported Reactivity | Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human IL-3Rα transfected COS cells. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Blue Laser (488 nm) |
|---|
| Application Notes | Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Sun Q, et al. 1996. Blood 87:83. (IP, WB) Herling M, et al. 2003. Blood 101:5007. (IHC) Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC) |
|---|
| Product Citations | Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed |
|---|
| RRID | AB_2629569 (BioLegend Cat. No. 306035) AB_2629570 (BioLegend Cat. No. 306036) |
|---|
| Structure | Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD |
|---|
| Distribution | Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes |
|---|
| Function | Hematopoietic cell proliferation, differentiation |
|---|
| Ligand/Receptor | IL-3 |
|---|
| Cell Type | Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes |
|---|
| Biology Area | Immunology |
|---|
| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
|---|
| Antigen References | 1. Miyajima A, et al. 1993. Blood 82:1960. |
|---|
| Gene ID | 3563 |
|---|
| UniProt | View information about CD123 on UniProt.org |
|---|
| Clone | 6H6 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | IL-3Rα, IL-3 Receptor alpha |
|---|
| Isotype | Mouse IgG1, κ |
|---|
