Biolegend, 306312, Alexa Fluor® 488 anti-human CD150 (SLAM) Antibody
100tests
Catalog Number: 306312
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| Verified Reactivity | Human, Cynomolgus, Rhesus |
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| Reported Reactivity | African Green, Baboon |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Activated human PBMC |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes |
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| Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation4, and costimulation1,5 of IFN-gamma production and T cell proliferation. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306317 and 306318). |
| Application References(PubMed link indicates BioLegend citation) | Garcia V, et al. 2001. J. Immunol. 167:5719. (Costim) Vincent S, et al. 2002. J. Virol. 76:6121. Cocks B, et al. 1995. Nature 376:260. Sayos J, et al. 2001. Blood 97:3867. (IP) Aversa G, et al. 1997. J. Immunol. 158:4036. (Costim) Spencer M, et al. 2010. Am. J. Physiol Endocrinol Metab. 299:1016. PubMed |
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| Product Citations | Verma A, et al. 2021. Cell Rep. 37:109942. PubMed Barbero AM, et al. 2021. J Leukoc Biol. 109:257. PubMed Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed |
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| RRID | AB_2187945 (BioLegend Cat. No. 306312) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 70-95 kD |
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| Distribution | T subset (upregulated after activation), B cells, dendritic cells, endothelial cells |
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| Function | B cell costimulation, proliferation and Ig production |
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| Ligand/Receptor | CD150 (self ligand) |
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| Cell Type | B cells, Dendritic cells, Endothelial cells, T cells, Tregs |
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| Biology Area | Costimulatory Molecules, Immunology, Innate Immunity |
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| Molecular Family | Adhesion Molecules, CD Molecules |
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| Antigen References | 1. Cocks B, et al. 1995. Nature 376:260. 2. Pinchouk V, et al. 1988. AntiCancer Res. 8:1377. 3. Polacino P, et al. 1996. J. Med. Primatol. 25:201. 4. Punnonen J, et al. 1997. J. Exp. Med. 185:993. |
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| Gene ID | 6504 |
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| UniProt | View information about CD150 on UniProt.org |
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| Clone | A12 (7D4) |
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| Regulatory Status | RUO |
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| Other Names | SLAM, Signaling Lymphocyte Activation Molecule, IPO-3 |
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| Isotype | Mouse IgG1, κ |
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