Biolegend, 306510, APC anti-human CD184 (CXCR4) Antibody
100tests
Catalog Number: 306510
Loading…
| Verified Reactivity | Human, Cynomolgus, Rhesus |
|---|---|
| Reported Reactivity | African Green, Baboon, Chimpanzee, Sooty Mangabey |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | CP-MAC-infected Sup-T1 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. |
| Excitation Laser | Red Laser (633 nm) |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Application Notes |
|---|
| Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540). |
| Application References(PubMed link indicates BioLegend citation) | McKnight A, et al. 1997. J. Virol. 71:1692. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF) Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC) Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block) Ullrich CK, et al. 2000. Blood 96:1438. (Block) Murga M, et al. 2005. Blood 105:1992. (IF) Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed Isnardi I, et al. 2010. Blood 115:5026. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Fischer T, et al. 2008. PLoS One 3:e4069. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC) |
|---|
| Product Citations | Gao Y, et al. 2016. Nat Methods. 10.1038/nmeth.4042. PubMed Matkovic Leko I, et al. 2023. Nat Protoc. . PubMed Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed Lu D, et al. 2022. ACS Synth Biol. 11:1397. PubMed Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Xu X, et al. 2021. Molecular Cell. :. PubMed Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed Isnardi I, et al. 2010. Blood. 115:5026. PubMed Gludish DW, et al. 2020. Sci Rep. 10:19900. PubMed Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed Agha O, et al. 2020. JOR Spine. 3:e1091. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed Hyun Hee Lee, Vanessa Bellat, Benedict Law 2017. PLoS One. 12(2):e0171044. PubMed Huijbregts R, et al. 2013. Endocrinology. 154:1282. PubMed Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed Cai J, et al. 2021. eLife. 10:00. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Kempton HR, et al. 2020. Mol Cell. 184:78. PubMed Fiedorowicz M, et al. 2020. Sci Rep. 10:5379. PubMed Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed Toyoda M, et al. 2015. J Virol. 89: 9639-9652. PubMed Shi J, et al. 2012. Blood. 120:1624. PubMed Meng YM, et al. 2018. Oncoimmunology. 7:e1408745. PubMed Walker KL, et al. 2021. Leuk Lymphoma. 62:1167. PubMed Grosche L, et al. 2020. Viruses. 12:. PubMed Blanco-Camarillo C, et al. 2021. Front Immunol. 12:672520. PubMed Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed Nakamura M, et al. 2019. Nat Commun. 10:194. PubMed Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed Shan L, et al. 2017. Immunity. 47:766. PubMed Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed |
|---|
| RRID | AB_314615 (BioLegend Cat. No. 306509) AB_314616 (BioLegend Cat. No. 306510) |
|---|
| Structure | Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD |
|---|
| Distribution | T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells |
|---|
| Function | B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development |
|---|
| Ligand/Receptor | SDF-1 receptor, coreceptor for X4 HIV-1 |
|---|
| Cell Type | B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs |
|---|
| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells |
|---|
| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors, GPCR |
|---|
| Antigen References | 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657. 2. Loetscher P, et al. 2000. Adv. Immunol. 74:127. 3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145. |
|---|
| Gene ID | 7852 |
|---|
| UniProt | View information about CD184 on UniProt.org |
|---|
| Clone | 12G5 |
|---|
| Regulatory Status | RUO |
|---|
| Workshop | VII 70204 |
|---|
| Other Names | CXCR4, Fusin |
|---|
| Isotype | Mouse IgG2a, κ |
|---|
