Biolegend, 308108, Alexa Fluor® 488 anti-human Perforin Antibody
100tests
Catalog Number: 308108
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| Verified Reactivity | Human |
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| Reported Reactivity | Cow |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified granules from the human lymphoma cell line |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Blue Laser (488 nm) |
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| Application Notes | Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.Does not cross-react with mouse1. |
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| Application References(PubMed link indicates BioLegend citation) | Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) Schaerli P, et al. 2004. J. Exp. Med. 199:1265. Watanabe N, et al. 1997. Blood 90:3662. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC) Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF) Chen H, et al. 2005. J. Immunol. 175:591. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF) Wood SM, et al. 2009. Blood 114:4117. PubMed Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798. |
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| Product Citations | Kennedy PR, et al. 2023. Front Immunol. 14:1060905. PubMed Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed Orange E 2014. Proc Natl Acad Sci U S A. 111:6708. PubMed Kabanova A, et al. 2016. Cell Rep. S2211-1247: 30219-4. PubMed Ambrose AR, et al. 2020. Proc Natl Acad Sci U S A. 117:23717. PubMed Davey M, et al. 2017. Nat Commun. 10.1038/ncomms14760. PubMed Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed Salzer E, et al. 2016. Nat Immunol. 17:1352-1360. PubMed Khazen R, et al. 2016. Nat Commun. 7:10823. PubMed |
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| RRID | AB_493252 (BioLegend Cat. No. 308108) |
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| Structure | 70 kD |
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| Distribution | CTL, NK (cytoplasmic granules) |
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| Function | Mediates targeted cell lysis |
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| Cell Type | NK cells, T cells |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735. |
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| Gene ID | 5551 |
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| UniProt | View information about Perforin on UniProt.org |
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| Clone | dG9 |
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| Regulatory Status | RUO |
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| Other Names | PRF1, P1, PFP, HPLH2 |
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| Isotype | Mouse IgG2b, κ |
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