Biolegend, 309242, Alexa Fluor® 647 anti-human CD2 Antibody
100tests
Catalog Number: 309242
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Additional reported applications (for the relevant formats) include: blocking of T cell activation, and partial blocking of B cell costimulation2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 309235 & 309236). |
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| Application References(PubMed link indicates BioLegend citation) | Schlossman S, et al. Eds.1995. Leucocyte Typing V Oxford University Press. New York. Hughes CCW, et al. 1996. J. Biol. Chem. 271:5369. |
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| RRID | AB_3716896 (BioLegend Cat. No. 309241) AB_3716896 (BioLegend Cat. No. 309242) |
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| Structure | Ig superfamily, type I transmembrane glycoprotein, 50 kD |
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| Distribution | T cells, NK cells, thymocytes, and thymic B cell subsets |
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| Function | T cell activation, adhesion |
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| Ligand/Receptor | CD58 (LFA-3), CD48, CD59, CD15 |
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| Cell Type | B cells, NK cells, T cells, Thymocytes |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Bell G, et al. 1995. J. Immunol. 155:2805. 2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Moingeon P, et al. 1989. Immunol. Rev. 111:111. |
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| Gene ID | 914 |
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| UniProt | View information about CD2 on UniProt.org |
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| Clone | TS1/8 |
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| Regulatory Status | RUO |
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| Workshop | V S025 |
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| Other Names | LFA-2, T11, SRBC-R |
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| Isotype | Mouse IgG1, κ |
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