Biolegend, 309416, Alexa Fluor® 647 anti-human CD275 (B7-H2, ICOSL) Antibody
100tests
Catalog Number: 309416
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Human B7H2-mIg fusion protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application References(PubMed link indicates BioLegend citation) | Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563. |
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| RRID | AB_2810465 (BioLegend Cat. No. 309415) AB_2810466 (BioLegend Cat. No. 309416) |
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| Structure | Ig superfamily, B7 family, 40 kD |
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| Distribution | B cells and dendritic cells |
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| Function | Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production |
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| Ligand/Receptor | ICOS |
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| Cell Type | B cells, Dendritic cells |
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| Biology Area | Costimulatory Molecules, Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | Wang S, et al. 2002. J. Biol. Chem. 96:2808. Wong SC, et al. 2003. Blood 102:1831. |
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| Gene ID | 23308 |
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| UniProt | View information about CD275 on UniProt.org |
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| Clone | 2D3 |
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| Regulatory Status | RUO |
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| Other Names | CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1 |
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| Isotype | Mouse IgG2b, κ |
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